FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010973
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT2350.036.19591532
CGTATGC1400.034.5700241
AATCGCG501.9438643E-434.2459136
TCGTATG1550.033.38726840
GCGAATC2450.033.37467233
GTATCAA31000.032.804331
GGTATCA15600.032.702331
TAAGGCG2800.031.4825229
ACGAGAC2850.030.83530422
TCTCGTA1650.029.17855638
CTCGTAT1750.027.5472739
GACTAAG3200.027.50488326
GCGATTA500.00842048427.12340229
ACTAAGG3450.026.50219227
ATCAACG38600.026.346753
CTTATAC3600.026.2773721
TATCAAC39100.026.1813642
AACGCAG38800.026.1238636
TCAACGC39150.026.0629184
CAACGCA39500.025.7464455
Produced by FastQC (version 0.11.5)