FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871199
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80050.041.7358741
GGTATCA33100.038.471221
GTGGTAT10800.037.7176131
TATCAAC90900.036.94152
ATCAACG97250.034.705373
TCAACGC97500.034.6533434
CAACGCA98050.034.5287865
ATTTACG750.002748593234.10090670
AACGCAG100650.033.6386346
TGGTATC12850.031.4331782
ACGCAGA107700.031.338797
CTCGTAT3350.030.9608739
CGCAGAG109450.030.868998
AGAGTAC108300.030.75262511
CAGAGTA109500.030.44849610
GCAGAGT114100.029.6109649
TCGTATG4450.028.00164640
AGTACGG80600.026.83840213
GAGTACG82100.026.47453712
CGTATGC4650.026.08674241
Produced by FastQC (version 0.11.5)