FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999056
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA306.6145527E-445.5635954
GTATCAA37250.038.8991931
GGTATCA16550.034.2776571
ACGCGTA1500.034.22237827
TATCAAC42650.033.972392
TCAACGC43950.033.1230244
GACGCGT1550.033.1101326
AACGCAG44400.032.635026
ATCAACG44800.032.4945763
CAACGCA44600.032.4120525
TATACGG450.004843021330.3757322
CGCATTA450.004845393430.37269217
ACGCAGA47900.030.1745417
TCGTATG1501.8189894E-1230.0160140
TATCGTC604.7235715E-429.43498657
AGAGTAC48550.029.20751811
CGCAGAG49500.028.9921138
CAGAGTA48950.028.96884510
GCAGAGT51950.027.6905989
CGCGTAG1900.027.02715128
Produced by FastQC (version 0.11.5)