FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999056
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21750.21770551400522092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38850.040.278341
TATCAAC44850.034.7963182
ATCAACG46600.033.2547573
TCAACGC47100.032.8934974
AACGCAG48000.032.6322176
CAACGCA47750.032.5886655
GGTATCA15600.031.3190461
ACGCAGA51750.030.2675637
CGCAGAG52050.030.0931138
GTGGTAT6900.029.7097661
CAGAGTA52650.029.68535410
AGAGTAC52500.029.6401711
ATAAACG704.1413423E-529.2574863
GCAGAGT54400.028.6676719
TGGTATC7550.027.5906812
ACGGTAT1300.001210021925.05513870
AGTACGG42150.025.01704413
GAGTACG42900.024.8978712
TACGGGG18050.023.25661515
GAGTACT21950.021.765512
Produced by FastQC (version 0.11.5)