FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430419
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37180.2599238404970851No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA30870.2158108917736691No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG28380.19840340487647326No Hit
GTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGCCATTACCAACTATGTAAATTGGTATCAGCAGAAA26980.18861606284592136No Hit
GTCTCTGGTGGCTCCATCACCAATTACTACTGGAGCTGGATCCGGCAGCC25850.18071627963554734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25180.17603233737806895No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24430.17078911843313044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23860.16680427203497716No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23800.16638481451938208No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22340.1561780149732351No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22140.15477982325458484No Hit
GGCCAAAGGTGTACGGGACATTATCAAACTGTTGACAGTAATATGTTGCA22080.15436036573898976No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21920.15324181236406956No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCA21830.15261262609067694No Hit
GTCTATTACTGTGCGGGAGGTTGGCGCTGTGGTGGTGGTACCTGCTACTC21060.1472295879738734No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20880.14597121542708813No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20860.14583139625522312No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGCCATTACCAA20150.14086781565401466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19790.13835107056044418No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT19320.13506532002161606No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18800.13143002155312533No Hit
TTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATT18290.12786463267056714No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG17990.12576734509259174No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17890.12506824923326662No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17840.12471870130360405No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17690.12367005751461634No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16630.11625964140576993No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15970.11164560873422402No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATTCCAGATCCACTTCCA15900.11115624163269644No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATTCCAGATCCACTTCCA15370.10745103357827322No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT15280.10682184730488059No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA15250.10661211854708305No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15150.10591302268775792No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC15000.10486437889877022No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14930.10437501179724262No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14720.10290691049265985No Hit
GTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGG14620.10220781463333471No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14330.10018043664129182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28750.040.7172551
TATCAAC32050.036.4186862
GTGGTAT3450.035.926991
ATCAACG33800.034.8387033
TCAACGC33950.034.7886284
TCGTATG601.3245239E-534.56900440
CAACGCA34300.034.4360545
AACGCAG35000.033.7473266
TGGGTTG13750.031.21005870
AGAGTAC38150.030.86519411
ACGAGAC1359.094947E-1230.60661322
CAGAGTA39250.029.9145510
CGCAGAG39400.029.8891018
GCAGAGT40100.029.4521879
TATTTAG3650.028.9408470
ACGCAGA41250.028.7155727
CACTGAT11500.026.9826170
CGTATGC809.409641E-525.93676841
TGTTAGA6250.025.35217370
AGTACGG31950.024.89133613
Produced by FastQC (version 0.11.5)