FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430419
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37920.2650971498560911No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA29330.20504481554006201No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG25890.18099591797927742No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25690.17959772626062714No Hit
GTCTCTGGTGGCTCCATCACCAATTACTACTGGAGCTGGATCCGGCAGCC23940.16736354872243728No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23920.16722372955057224No Hit
GTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGCCATTACCAACTATGTAAATTGGTATCAGCAGAAA23050.16114159557444357No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22870.15988322302765834No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22410.15666738207476272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21370.1493967851377813No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20920.1462508537708182No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCACACGAACTGTGGCTGCACCA20840.14569157708335811No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20840.14569157708335811No Hit
GTCTATTACTGTGCGGGAGGTTGGCGCTGTGGTGGTGGTACCTGCTACTC20230.14142709234147477No Hit
GGCCAAAGGTGTACGGGACATTATCAAACTGTTGACAGTAATATGTTGCA20120.14065808689621712No Hit
TTATCAAACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATT18460.1290530956314199No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGCGTCACCATCACTTGCCAGGCGAGTCAGGCCATTACCAA18290.12786463267056714No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17770.12422933420207646No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17710.12380987668648138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17400.12164267952257345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17360.12136304117884339No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAGCTTGGTCCCCTGGCCAAAGGTG17250.12059403573358575No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATTCCAGATCCACTTCCA16360.11437208258559205No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16340.11423226341372704No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16190.11318361962473933No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16010.11192524707795408No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT15940.11143587997642648No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGA15540.10863949653912594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15500.10835985819539591No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15160.10598293227369045No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14770.10325645842232242No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAACTCTATTCCAGATCCACTTCCA14690.10269718173486231No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14490.10129899001621204No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14350.10032025581315684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)