FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063550
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAACTTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA87730.8248789431620517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44750.42076066005359414No Hit
GTTGTATACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCCCCACCCGGCTGATTTTCAGTGTGAAATCTG43060.40487048093648625No Hit
CTCCTGCATAGTGATGGAAAGACCCATTTGTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAAT36850.3464811245357529No Hit
GTGATGGAAAGACCCATTTGTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTT30260.28451882845188287No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29690.2791594189271778No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29580.27812514691363827No Hit
GTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTC28950.2722015890179117No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28310.26618400639368156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27420.25781580555686145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26690.25095200037609894No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26360.24784918433548025No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23030.2165389497437826No Hit
GTCTGGCACTCCGGAGAACCGGCTGGAAACTTGAAAGATTAGGAGATGTGGAGACTGGCCTGGCTTCTGCACGTA22070.20751257580743734No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21360.20083682008368203No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21160.1989563255136101No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20400.19181044614733675No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG17930.16858633820694843No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17600.16548352216632975No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGA17590.16538949743782613No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17320.16285082976822904No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17150.16125240938366792No Hit
GTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTTCCAG17110.1608763104696535No Hit
ATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGAGAGTGGAGT16950.15937191481359597No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACTCTCT16890.1588077664425744No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATC16840.1583376428000564No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15590.14658455173710686No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15320.14404588406750976No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15210.14301161205397017No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15180.1427295378684594No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15100.14197734004043064No Hit
GTGCCAGACAGGTTCAGTGGCAGCGGGTCAGGGGCAGATTTCACACTGAA14800.13915659818532275No Hit
CCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGACTCTCCTG14790.13906257345681913No Hit
AGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTTCCAGCCGGTTC14760.13878049927130834No Hit
TTCCATCACTATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCC14740.13859244981430116No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACT14730.13849842508579757No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14270.13417328757463215No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14250.13398523811762492No Hit
GTCTCTGGTGGCTCCATCAGCAGTGCTGATTACTACTGGAGTTGGATCCG14140.13295096610408538No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG14020.13182266936204223No Hit
TACCAATACAAATGGGTCTTTCCATCACTATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGC14010.13172864463353862No Hit
GCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACTCTCTCTGTCCGTCACCC13620.12806168022189834No Hit
TTATTACTGCATGCAAGGTATACAACTTCCCATCACCTTCGGCCAAGGGA13610.12796765549339476No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13540.1273094823938696No Hit
CTCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA13530.127215457665366No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13360.12561703728080487No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC13300.12505288890978328No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13230.1243947158102581No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13030.12251422124018617No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12860.12091580085562502No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12750.11988152884208547No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12550.11800103427201354No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12530.11781298481500634No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12500.11753091062949556No Hit
GTAATAAACCCCAATATCCTCAGCCCCCACCCGGCTGATTTTCAGTGTGAAATCTGCCCCTGACCCGCTGCCA12490.11743688590099197No Hit
GTCTCCACATCTCCTAATCTTTCAAGTTTCCAGCCGGTTCTCCGGAGTGC12350.11612053970194161No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12130.1140519956748625No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12040.11320577311833013No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11880.11170137746227259No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11770.11066710544873302No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11660.10963283343519345No Hit
GTTCTCCGGAGTGCCAGACAGGTTCAGTGGCAGCGGGTCAGGGGCAGATT11550.1085985614216539No Hit
GGTCAGGGGCAGATTTCACACTGAAAATCAGCCGGGTGGGGGCTGAGGAT11230.1055897701095388No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11060.10399134972497767No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11050.10389732499647407No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10840.10192280569789854No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10820.10173475624089136No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10670.10032438531333741No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10660.10023036058483381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAA400.00854662149.8244970
ACTAGCG1500.045.85974527
CTAGCGC1600.043.0036328
GGTATCA6800.042.461731
CTGTCTT32050.041.6629170
GTATCAA15950.039.438261
TAGCGCT1750.039.33611729
TAATGCG350.001371957239.2861721
GACTAGC1950.037.03708326
AGACTAG2050.036.90455625
TATCAAC18750.033.732142
GTGGTAT2250.033.609921
TACACAT2150.033.574395
CTTATAC2200.032.811341
CTCGTAT958.62201E-932.7509939
CGTATGC751.6299819E-632.2978741
ATCAACG20150.031.5590553
TATACAC2400.031.50933
CAACGCA20250.031.403215
GAGACTA2300.031.39804624
Produced by FastQC (version 0.11.5)