FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063550
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAACTTCCCATCACCTTCGGCCAAGGGACACGACTGGAGATTAAA86530.8135959757416201No Hit
GTTGTATACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCCCCACCCGGCTGATTTTCAGTGTGAAATCTG52270.4914672558882986No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44210.41568332471439995No Hit
CTCCTGCATAGTGATGGAAAGACCCATTTGTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAAT33100.3112218513469042No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28230.26543180856565274No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28000.26326923981007005No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27140.25518311315876074No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26510.2492595552630342No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26100.24540454139438672No Hit
GTGATGGAAAGACCCATTTGTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTT26070.24512246720887593No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24060.22622349677965306No Hit
GTATTGGTACGTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTC23030.2165389497437826No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22400.21061539184805603No Hit
GTCTGGCACTCCGGAGAACCGGCTGGAAACTTGAAAGATTAGGAGATGTGGAGACTGGCCTGGCTTCTGCACGTA21500.20215316628273233No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20780.1953833858304734No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20730.19491326218795543No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20300.1908701988623008No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19740.18560481406609938No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG19420.18259602275398432No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17820.16755206619340887No Hit
GTAATAAACCCCAATATCCTCAGCCCCCACCCGGCTGATTTTCAGTGTGAAATCTGCCCCTGACCCGCTGCCA17380.16341497813925063No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16600.15608104931597008No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16590.15598702458746652No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16550.1556109256734521No Hit
GATCAGGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATC15780.14837102157867518No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15440.1451741808095529No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15250.14338771096798458No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGA15120.14216538949743782No Hit
GTCTCTGGTGGCTCCATCAGCAGTGCTGATTACTACTGGAGTTGGATCCG15020.14122514221240187No Hit
ATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGCCCAGGGGTGACGGACAGAGAGAGTGGAGT14940.14047294438437308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14680.13802830144327957No Hit
GCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACTCTCT14520.13652390578722204No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14420.1355836585021861No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14170.13323304028959618No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14100.13257486719007097No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTTCTCAGTTCATCTTCTCACC14080.1323868177330638No Hit
GTGCAGAAGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTTCCAG13940.13107047153401347No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13430.12627521038033002No Hit
TTATTACTGCATGCAAGGTATACAACTTCCCATCACCTTCGGCCAAGGGA13150.12364251798222933No Hit
TTCCATCACTATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCC13110.12326641906821494No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13040.12260824596868976No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13000.12223214705467539No Hit
AGCCAGGCCAGTCTCCACATCTCCTAATCTTTCAAGTTTCCAGCCGGTTC12810.12044567721310705No Hit
GGACTTCTCAGTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG12670.1191293310140567No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12640.11884725682854591No Hit
GTTCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT12610.11856518264303513No Hit
CAGTAATAAACCCCAATATCCTCAGCCCCCACCCGGCTGATTTTCAGTGTGAAATCTGCCCCTGACCCGCTGCCA12560.11809505900051713No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACT12360.11621456443044521No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12270.11536834187391284No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12060.11339382257533731No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11980.11264162474730856No Hit
TACCAATACAAATGGGTCTTTCCATCACTATGCAGGAGAGTCTGACTAGACTTGCAGGAGATGGAGGCCGGCTGC11940.11226552583329416No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11870.11160735273376898No Hit
GTCGTGTCCCTTGGCCGAAGGTGATGGGAAGTTGTATACCTTGCATGCAGTAATAAACCCCAATATCCTCAGCCC11830.1112312538197546No Hit
GTGCCAGACAGGTTCAGTGGCAGCGGGTCAGGGGCAGATTTCACACTGAAAATCAGCCGGGTGGGGGCTGAGGAT11830.1112312538197546No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11680.10982088289220066No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11670.10972685816369705No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11440.10756428940811433No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11370.10690611630858916No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11340.10662404212307837No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11300.10624794320906399No Hit
GTTCTCCGGAGTGCCAGACAGGTTCAGTGGCAGCGGGTCAGGGGCAGATT11040.10380330026797048No Hit
GGTCAGGGGCAGATTTCACACTGAAAATCAGCCGGGTGGGGGCTGAGGAT11000.10342720135395608No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10870.10220487988340933No Hit
CTCTCTGTCCGTCACCCCTGGGCAGCCGGCCTCCATCTCCTGCAAGTCTA10870.10220487988340933No Hit
GCTAATGCTCTGGATCCCTGGATCCAGTGCGGATATTGTGATGACTCAGACTCCACTCTCTCTGTCCGTCACCC10830.10182878096939496No Hit
GTGCCAGACAGGTTCAGTGGCAGCGGGTCAGGGGCAGATTTCACACTGAA10690.10051243477034459No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10650.10013633585633022No Hit
GGTGGGGGCTGAGGATATTGGGGTTTATTACTGCATGCAAGGTATACAAC10640.10004231112782662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)