FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025303
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58300.038.3846781
TATCAAC67200.033.300172
CTCGTAT3450.031.94279539
ATCAACG71050.031.590883
TCAACGC71100.031.568664
TCGTATG3500.031.55870240
GGTATCA29350.031.4429261
CAACGCA71300.031.433485
AACGCAG73500.030.53866
CGTATGC3700.028.98422841
GTGGTAT10300.028.2247221
ACGCAGA81450.027.4319927
AGAGTAC81500.026.959611
CGCAGAG83100.026.9286568
CAGAGTA84050.026.30254410
GCAGAGT87600.025.3909829
TATATCG1201.0384247E-725.353025
AGTACGG54000.023.34866513
GAGTACG55600.022.67676212
CTTATAC5600.022.3348031
Produced by FastQC (version 0.11.5)