FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2025303
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39120.19315628328205706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63500.038.322571
GTGGTAT10300.036.7516331
GGTATCA30350.034.5222321
TATCAAC70750.034.1765562
ATCAACG75500.031.745453
TCAACGC75950.031.4661354
CAACGCA76700.031.202465
AACGCAG79350.030.245496
ACGCAGA86600.027.8303227
CGCAGAG86350.027.7545348
AGAGTAC87100.027.4373611
CAGAGTA89450.026.64106410
GCAGAGT92300.025.9653769
TGGTATC15050.024.9073872
AGTACGG56750.022.77977613
GAGTACG58400.022.0211212
TACGGGT12650.021.88070315
GAGTACT50450.021.87831112
AGTACTT50100.021.76112213
GTACTTT51950.020.98618514
Produced by FastQC (version 0.11.5)