FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648273
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33030.20039156134936384No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22630.13729521747914333No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGAATACTACTGGAGTTGGATCCG22350.13559646975956047No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG21940.13310901774159983No Hit
GAGTTACAGTACCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT21830.13244165256604945No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21110.12807344414426494No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGTGACAGATTTCA20840.12643536598609575No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT20590.12491862695075391No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20220.12267385317844799No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19560.11866966212514553No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18360.1113893147555047No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18090.1097512365973355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17900.10859851493047572No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17830.10817382800058001No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17810.10805248887775266No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16800.10192486317497162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5000.037.882821
GGTATCA13050.037.2098771
GTATCAA25600.035.78421
TATCAAC28350.032.4355282
TGGTATC6000.030.9959752
TCAACGC29300.030.6804714
ATCAACG30050.030.2567483
CAACGCA30400.029.5703225
AACGCAG31100.029.016386
AGAGTAC35250.025.69252211
ACGCAGA35050.025.6449647
TGTTAGA5850.025.57417570
CGCAGAG35250.025.4994628
TCGTATG1001.5318516E-524.21524440
ACCGTAC1151.714785E-623.97351827
CAGAGTA38100.023.77208310
GCAGAGT38500.023.7039979
AGTACGG30800.021.91302113
GAGTACG31600.021.57685912
CAGCGCT5500.019.41284
Produced by FastQC (version 0.11.5)