FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648273
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31910.1935965704710324No Hit
GAGTTACAGTACCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT22330.1354751306367331No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG22140.1343224089698733No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGTGACAGATTTCA21660.13141027002201697No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGAATACTACTGGAGTTGGATCCG21540.1306822352850529No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20480.1242512617752035No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19190.11642488835283962No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18950.11496881887891144No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18890.11460480151042941No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT18080.10969056703592185No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18060.10956922791309451No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17930.10878052361471674No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17790.10793114975492529No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17100.10374495001738183No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAACACCTATTTAAATTGGTTTCAGCAGAAA16870.10234955010486733No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16590.10065080238528448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27850.037.721251
GGTATCA13150.037.1940961
GTGGTAT5450.036.023821
TATCAAC30050.034.5905382
ATCAACG31900.032.45693
TCAACGC32300.032.0510674
CAACGCA32950.031.4178495
AACGCAG33650.031.0709066
TGGTATC6450.028.2820592
CGCAGAG38550.027.4784168
AGAGTAC38250.027.33427411
ACGCAGA38950.026.8430257
GCAGAGT41700.025.402719
CAGAGTA42350.024.9316110
GTATGTA9100.024.2242281
AGTACGG33500.022.89419713
GAGTACG34650.022.63064812
TATGTAC8850.022.5569082
TTCGGGG12900.021.96301870
GTACACG8900.021.6403065
Produced by FastQC (version 0.11.5)