FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC18740.2469304984201192No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17690.23309501158227897No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAAAGCCTCGTA14070.18539552362705852No Hit
GGTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT13590.17907072964404586No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13290.17511773340466297No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13210.17406360107416083No Hit
GTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAA12890.1698470717521524No Hit
GTGTATTACTGTACGAAACATTCCCCGTCGGGGCAGCTGATTCTTGAATC11460.1510044563444272No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11390.15008209055523786No Hit
GTCTCTAGTGGCTCCGTCAGGAATAGTTTTTACTACTGGGGCTGGATCCG11320.14915972476604852No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10780.14204433153515927No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10730.14138549882859547No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTTAGGGAGA10650.14033136649809333No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10430.13743250258921252No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10290.13558777101083383No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10240.13492893830427002No Hit
AGGTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT10230.13479717176295727No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG9950.13110770860619989No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9820.12939474356913394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9660.12728647890812975No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA9220.12148875109036814No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9060.11938048642936391No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8790.1158227898139193No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC8710.11476865748341718No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8670.11424159131816614No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8280.10910269620696834No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8090.10659913192202583No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8070.10633559883940032No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7990.10528146650889819No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7760.10225083605870462No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC7750.10211906951739186No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7630.10053787102163869No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG7620.10040610448032593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14750.039.3252451
GGTATCA6300.039.2255171
TAGGCAA1852.3283064E-1036.1012270
TACGGAT5650.035.46225770
TAACTAG400.002661478234.324595
TATCAAC17100.034.3223272
GTGGTAT2300.034.3223271
TCAACGC17600.033.5444834
ATCAACG17650.033.4494553
TTCGGGG6550.032.6288970
CAACGCA18250.032.537835
AACGCAG18600.031.5585586
TCGTATG1001.4144462E-831.12599240
GTACGGA550.004620770530.6670569
CTTATAC1700.030.2844051
AGAGTAC20200.028.87941611
CGAGACA1950.028.16376723
CAGAGTA20850.027.979110
CGCAGAG21100.027.9747058
ACGAGAC1850.027.82708222
Produced by FastQC (version 0.11.5)