FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC20870.2749967717197378No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16780.2211042563228175No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15460.20371107286953266No Hit
GTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAA13130.17300946874365875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12720.16760704054983544No Hit
GGTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT12270.16167754619076108No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11950.1574610168687526No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11850.156143351455625No Hit
GTGTATTACTGTACGAAACATTCCCCGTCGGGGCAGCTGATTCTTGAATC11670.15377155371199522No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11350.1495550243899868No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCCAGTCAAAGCCTCGTA11270.1485008920594847No Hit
GTCTCTAGTGGCTCCGTCAGGAATAGTTTTTACTACTGGGGCTGGATCCG11180.14731499318766983No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10760.14178079845253375No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTTAGGGAGA10560.13914546762627847No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10250.13506070484558277No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCAGGGCCGAAAGTG10040.13229360747801477No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10020.13203007439538925No Hit
AGGTACACACTGGCCTTTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT9860.129921809734385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9540.12570528041237655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9290.12241111687955747No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC8650.11397805823554061No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG8560.11279215936372572No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8310.10949799583090662No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA8280.10910269620696834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8250.10870739658303005No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC8230.10844386350040452No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7850.1034367349305195No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7810.10290966876526846No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA7770.10238260260001739No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7740.1019873029760791No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC7700.10146023681082805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.042.2558751
GTATCAA14750.040.5476841
CAACGCA17700.033.914525
ATCAACG17500.033.720743
AACGCAG18050.033.6369746
TATCAAC17850.033.2714922
TCAACGC17800.033.1458664
TACGGAT4050.031.52015770
CTTATTA450.004249200731.19784759
AGAGTAC19900.031.02703911
CGCAGAG20150.029.7909188
CAGAGTA20600.029.63969210
GCAGAGT21050.028.6801539
ATCAGTG11550.028.50387470
GATCCGA500.0071913728.01385556
ACGCAGA21600.027.9498717
TATAAGA1653.6379788E-1227.047292
TATATGT809.938744E-525.7265854
AGTACGG17850.025.3662713
GTATAAA951.0807878E-525.326971
Produced by FastQC (version 0.11.5)