FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859125
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16440.191357485814055No Hit
GTGTATTACTGTGCGAGGGACATAGGATATTGTAGTGATCCCAGTTGCTACGCGGACTGGTTCGACCCCTGGGGC13430.15632183908045977No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11270.13117997963043795No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10710.12466171977302488No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA9900.11523352247926669No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9460.11011203259129929No Hit
GTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT9030.10510694020078569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.040.9654541
GTATCAA17100.036.970151
TATCAAC19800.031.9027272
TTATGCG450.004751220330.494349
TATCCGT450.004751220330.494345
TCAACGC20750.030.255534
ATCAACG20800.030.1845593
CAACGCA21500.029.3596695
AACGCAG21550.029.291556
GTGGTAT3250.028.5436741
ACGCAGA23750.026.8671197
AGAGTAC23950.026.6427611
CGCAGAG24550.026.2710938
CAGAGTA24550.026.13135310
GCAGAGT25400.025.3919439
AGTACGG21100.023.90048213
GAGTACG21450.023.67043512
TAGGCAA2852.3799657E-823.41490670
GTATAAG2500.023.3635251
TGGTATC4100.022.6076322
Produced by FastQC (version 0.11.5)