FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203968
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19980.16595125451839252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31700.035.1001431
TATCAAC36100.030.3198722
TCAACGC36350.030.0888394
ATCAACG36600.029.790873
CAACGCA37000.029.560255
AACGCAG37350.029.5586366
GTGGTAT7700.028.9898381
GGTATCA16150.028.494131
ACGCAGA40400.027.4119727
AGAGTAC40450.026.5304711
CGCAGAG42600.025.9158468
CAGAGTA41300.025.90142610
TGGTATC8600.025.135412
GCAGAGT44600.024.8305789
TTGCGAC700.001383083324.49013112
TCTGTCG2001.8189894E-1224.000338
CGAATTA1001.6408525E-524.0003315
GAGTACG35900.021.9660812
AGTACGG35600.021.95856713
AATAGCG2950.021.52743162
Produced by FastQC (version 0.11.5)