FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320240
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT51760.39204993031570023No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACT47140.3570562927952493No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44140.33433315154820337No Hit
GTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAACAGAA37820.28646306732109317No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35080.2657092649821245No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33120.25086347936738773No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30780.23313942919469186No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28320.21450645337211413No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28100.2128400896806641No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27980.21193116403078227No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT27140.2055686844816094No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26580.2013270314488275No Hit
ATATTATAATGCTCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25680.19451008907471368No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25620.19405562624977277No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25140.19041992365024543No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA24390.1847391383384839No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24380.1846633945343271No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23670.17928558443919287No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC21980.1664848815366903No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21790.16504574925771073No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21550.16322789795794704No Hit
GTCCCTGACCGATTCCGGGGCAGCGGGTCTGGGACAGATTTCACTCTCAC21410.16216748469975156No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20880.1581530630794401No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20370.15429012906744227No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20330.15398715385081502No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19900.15073017027207175No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19800.14997273223050356No Hit
GTAAAATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19700.14921529418893534No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC19580.14830636853905352No Hit
GTCTGGTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC19420.1470944676725444No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19130.1448978973519966No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19010.14398897170211478No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18910.14323153366054658No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA18830.142625583227292No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18320.1387626492152942No Hit
GTTTATTACTGTCAGCAATATTATAATGCTCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17990.13626310367811914No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17720.13421802096588498No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA17450.13217293825365087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17400.13179421923286674No Hit
GTACCAGACCATGCCATAGTCCCCTCTCGCACAGTAAAATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTG17220.130430830758044No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17050.12914318608737804No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA16900.12800702902502575No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG16900.12800702902502575No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16870.1277797976125553No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16860.12770405380839847No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16630.12596194631279162No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGTTGGTA16470.12475004544628249No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC16440.12452281403381203No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAACAATAAGAACTA16270.12323516936314609No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTTACTGGATGACCTGGG16190.12262921892989154No Hit
ATTCCGGGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16020.12134157425922559No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15770.1194479791553051No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15360.11634248318487547No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15040.11391868145185723No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14990.11353996243107314No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14950.11323698721444588No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14910.11293401199781858No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGCTCCTCTAACTTT14750.11172211113130946No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14370.10884384657335029No Hit
CTCCAGAGACAACGCCAAGAACTCATTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATTTTA14320.10846512755256621No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG14320.10846512755256621No Hit
CCATAAAGCCAGATGGAAATGAGAAATACTATCTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG14140.10710173907774344No Hit
GTACCAACAGAAACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCTGA14070.10657153244864571No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13970.10581409440707751No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13920.1054353753862934No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13860.10498091256135249No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13620.1031630612615888No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC13460.10195116039507968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATA150.002429275368.4663241
ATCGCGT200.007371697651.72790537
TAATCGC306.359643E-445.9295435
TCGTATG2400.044.66789640
CTTATAC3750.043.8184471
CGTATGC2450.043.8049241
CTCGTAT2300.043.5662139
GCTAATC3150.042.596933
GTGGTAT3150.040.2103841
GTATCAA24800.040.168751
CGAGACT4000.039.3964923
ACGAGAC4000.039.3860422
GGTATCA10450.039.310811
ATCTCCG4100.039.23843410
CTAATCT2900.039.17608334
CCGTCTT2350.038.48580647
ATCTCGT2700.038.31696737
ATGCCGT2650.037.97916844
CACGAGA4000.037.6693221
CCCACGA4100.037.57156419
Produced by FastQC (version 0.11.5)