FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320240
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTGTATAAAACACT44670.3383475731685148No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT44300.3355450524147125No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41570.31486699387990064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34690.2627552566200085No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33530.25396897533781737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32440.24571290068472398No Hit
GTTTTATACAACTCCAACAATAAGAACTACTTAGCTTGGTACCAACAGAA32070.24291037993092165No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTT29820.22586802399563716No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29760.22541356117069625No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27360.20723504817305943No Hit
TTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC27040.20481124644004123No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27010.20458401502757073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26600.20147851905714115No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT26190.19837302308671148No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25480.1929952129915773No Hit
ATATTATAATGCTCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25280.1914803369084409No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23170.17549839423135188No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA23160.17542265042719507No Hit
GTCCCAGACCCGCTGCCCCGGAATCGGTCAGGGACCCCGGATTCCCGGGT22660.17163546021935405No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22210.16822698903229716No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20860.15800157547112645No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA20680.15663818699630372No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20590.1559564927588923No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19920.15088165788038538No Hit
GTCTGGTACTACTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC19840.1502757074471308No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19740.14951826940556262No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC19670.1489880627764649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19250.14580682300187844No Hit
GTTTATTACTGTCAGCAATATTATAATGCTCCTCTAACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA19110.14474640974368297No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC18990.14383748409380115No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18980.1437617402896443No Hit
GTCCCTGACCGATTCCGGGGCAGCGGGTCTGGGACAGATTTCACTCTCAC18930.1433830212688602No Hit
GTAAAATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18880.14300430224807612No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18760.14209537659819427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18290.13853541780282372No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17730.1342937647700418No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17480.1324001696661213No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16730.1267193843543598No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA16730.1267193843543598No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16610.12581045870447796No Hit
GTACCAGACCATGCCATAGTCCCCTCTCGCACAGTAAAATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTG16590.12565897109616433No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGTTACTGGATGACCTGGG16490.12490153305459613No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGTTGGTA16090.12187178088832334No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16080.12179603708416653No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA16040.12149306186753923No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCCCGGAATCGGTCAGGGACC15960.12088711143428468No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15940.12073562382597103No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15870.1202054171968733No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15800.11967521056777555No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15660.11861479730958008No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15440.11694843361813004No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15300.11588802035993456No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTCCTGGGCATCTACCCGGGAA14940.11316124341028903No Hit
ATTCCGGGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14860.11255529297703448No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAACTCCAA14820.11225231776040719No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG14680.11119190450221171No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14570.11035872265648669No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14560.11028297885232988No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14530.11005574743985942No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG14330.10854087135672302No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCCCGGAATCGGTCAGGGACCCCG14210.10763194570684118No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14120.1069502514694298No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13900.10528388777797976No Hit
CTCCAGAGACAACGCCAAGAACTCATTGTATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCTGTATTTTA13850.10490516875719567No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13790.10445070593225474No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGCTCCTC13770.1042992183239411No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTTAGAGGAGCATT13700.10376901169484336No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13500.10225413561170697No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAATGCTCCTCTAACTTT13430.10172392898260922No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG13390.10142095376598195No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13260.10043628431194328No Hit
GTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGTTG13220.10013330909531601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG200.0076155251.3039136
GTATCAA25200.040.0888751
GGTATCA10000.040.067131
GTGGTAT3150.038.050461
TATCAAC28000.035.688712
TGGTATC3400.034.2220542
ATCAACG29650.033.918083
TCAACGC29700.033.510384
AACGCAG30400.033.0775266
CAACGCA30550.032.6899645
CGCAGAG32650.030.9028198
AGAGTAC33200.030.08181411
GCAGAGT34100.029.4884689
CAGAGTA33800.029.34543410
ATAGTCT500.00635674828.7321566
GTGTATA1700.028.2021051
TAACGGC500.007782421527.56567636
AGAGTCG500.00806907227.36208711
ACGCAGA37250.027.0866327
GAGTACT14000.026.62917312
Produced by FastQC (version 0.11.5)