FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937143
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22650.034.610261
GGTATCA11050.033.1481321
GCGTATG652.4479394E-531.5991541
AACGTCG704.045562E-529.35773726
TCGTATG1103.5790436E-828.29369440
TATCAAC28050.028.069362
CAACGCA28200.027.8045985
AACGCAG28700.027.32026
TCAACGC28700.027.3172864
ATCAACG29350.026.7108753
CTCGTAT1057.592462E-726.3206139
CGCTTAT801.0072248E-425.67705324
TCGGTTT1300.001155263925.25452670
TAACGTC951.1131169E-525.23196425
GTGGTAT5700.025.2238861
ACGCAGA31650.024.6629547
TTACTCG700.001394688424.4543364
CGCAGAG32200.024.241698
AGAGTAC32300.024.05807911
ACGAGAC1852.0008883E-1124.05392822
Produced by FastQC (version 0.11.5)