FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421114
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCC37260.2621886773334159No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36550.25719259679378287No Hit
CAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33450.23537872401510365No Hit
GTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGG29150.2051207714511292No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28550.2008987315584816No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22890.1610708219045059No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT22840.16071898524678527No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT22470.15811539397965257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21970.15459702740244624No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCAGTGGCCCTGGT21850.15375261942391671No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21300.14988241618898976No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20820.14650478427487168No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20410.1436197236815625No Hit
ATGCTACATCCACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA19470.1370051945164146No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT19300.13580894988016443No Hit
GTGTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA19000.13369792993384064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18590.13081286934053143No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAAGGAGGCCACTGATTATA18540.1304610326828108No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18220.12820927807339874No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17630.12405760551229528No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17610.12391687084920702No Hit
CTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17300.12173548357133909No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16720.11765417834177976No Hit
GATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCC16670.11730234168405912No Hit
CTGATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGT16370.11519132173773532No Hit
GTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15710.11054707785582298No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15360.10808422125177854No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC15110.10632503796317537No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14450.101680794081263No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14330.10083638610273349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30150.040.0817151
TCGTATG1450.035.75424640
CGGTTGA400.002434933334.95498356
TATCAAC35000.034.233272
ATCAACG36250.033.147523
TCAACGC36400.033.0109184
CAACGCA37550.031.8170765
AACGCAG38150.031.2266866
CGTATGC1750.029.64595241
AGAGTAC40850.029.24369811
CGCAGAG41600.029.0465438
GTAAACG605.5782683E-428.61147919
AAACGTA500.00791047327.47378521
GCAGAGT43950.027.2591119
CAAACGA21000.026.99954470
ACGCAGA44400.026.9828347
CAGAGTA45200.026.50526210
TCGCTAT1551.6061676E-924.37533824
AGTACTT18050.023.9628513
AGTACGG32550.023.62338613
Produced by FastQC (version 0.11.5)