FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421114
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37200.26176647334415115No Hit
GTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCC32980.23207145943252971No Hit
CAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA30870.21722395247671897No Hit
GTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGG28750.2023060781893641No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA28280.19899881360679017No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25740.181125511394582No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20920.14720845759031295No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20750.1460122129540628No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20550.14460486632318026No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT20160.1418605403929593No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCAGTGGCCCTGGT20130.14164943839832694No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAAGGAGGCCACTGATTATA19640.13820143915266475No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT19490.13714592917950286No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19040.13397939926001715No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19000.13369792993384064No Hit
ATGCTACATCCACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA18990.1336275626022965No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18160.127787074084134No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17730.12476127882773654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17330.12194658556597149No Hit
GTGTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA17330.12194658556597149No Hit
CTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT17010.11969483095655943No Hit
CTGATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGT16310.11476911774847057No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT16050.11293956712832329No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15900.11188405715516138No Hit
GTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA15760.11089891451354358No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15440.10864715990413155No Hit
GATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCC15410.10843605790949917No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15120.1063954052947195No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15100.10625467063163124No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14910.10491769133229284No Hit
GTCTCTGGTGCTTCCATCAGCGGCGCTCATTACTACTGGAGTTGGATCCG14360.10104748809736587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33550.038.88781
TCAACGC39400.033.3341754
ATCAACG39650.033.213993
TATCAAC39500.033.1723062
CAACGCA39950.032.8752565
AACGCAG40400.032.5939526
TAACGGC2150.030.45417236
AGAGTAC42700.030.1958411
CGCAGAG44550.029.6346748
TTAACGG2100.029.52677335
ACGCAGA45450.028.97247
TGTTAGA7500.028.79343270
GCAGAGT46250.028.3229689
CAGAGTA46300.027.84799810
CGCGGTA2450.025.44988643
AGTACGG33900.025.18760113
GAGTACG34250.024.93020812
AACGGCC2700.024.26427337
TATTAGA3300.023.9069022
AGTACTT18350.023.73311813
Produced by FastQC (version 0.11.5)