FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014777
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26680.26291490642771764No Hit
GTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCC25970.2559182953496187No Hit
CAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA22000.2167963996030655No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20040.1974818112747924No Hit
GTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGG19480.19196335746671436No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15920.15688175825821832No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15700.15471379426218765No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15320.1509691291781347No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT14840.14623902591406782No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14630.14416960573603857No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14180.13973513392597586No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCAGTGGCCCTGGT14060.13855260810995915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13320.13126036557785603No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12920.12731861285780027No Hit
GTGTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12900.1271215252217975No Hit
ATGCTACATCCACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA12650.12465792977176267No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT12620.1243622983177585No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12380.12199724668572505No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAAGGAGGCCACTGATTATA12070.11894238832768185No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11810.11638024905964561No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11750.11578898615163727No Hit
GTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11400.11233995252158849No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11300.11135451434157455No Hit
CTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11260.11096033906956898No Hit
GATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCC11200.11036907616156061No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11100.10938363798154668No Hit
CTGATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGT10790.1063287796235035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCG901.10865425E-437.6726670
GTATCAA21300.037.34841
TATCAAC24250.033.0877882
ATCAACG25150.031.631053
TCAACGC25100.031.5590044
TATACAC2500.031.5465183
CAACGCA25850.030.5137185
AACGCAG25700.030.424936
TAAGACA2400.028.6552431
AATCCGC605.614374E-428.5789746
CGTATGC854.285419E-628.53102541
CGCAGAG27550.028.3776788
AGAGTAC28150.028.01506811
GTTATAG500.007968727527.4317571
CCCACGA2250.027.42905419
GCAGAGT29900.026.491379
ACGCAGA29650.026.2521447
TTATACA2750.026.1848582
CAAACGA15850.026.09752870
CAGAGTA30800.025.71727210
Produced by FastQC (version 0.11.5)