FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014777
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26470.26084548624968834No Hit
GTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCC24430.2407425473774041No Hit
CAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20870.20566094816890806No Hit
GTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGG20110.19817161800080216No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19260.1897953934706837No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18690.1841783958446043No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17420.1716633309584273No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTACAGTCTGAAGATT14310.14101620355999397No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14070.13865115192796051No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13800.1359904688419229No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13590.13392104866389365No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13150.12958512067183234No Hit
GTCCCAGACCCACTGCCACTGAACCTGGTTGGGATACCAGTGGCCCTGGT13060.12869822630981978No Hit
GTGTATTACTGTCACCAGTATAATCAGTGGCCTCCTTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAA12880.12692443758579472No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT12420.12239142195773062No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGAACTTAGCCTGGTATCAGCAGAAACCTGGCCAGGCTCCCAGGCT12210.12032200177970134No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACAAAGGAGGCCACTGATTATA12190.12012491414369858No Hit
ATGCTACATCCACCAGGGCCACTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCA12150.11973073887169298No Hit
GTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11760.11588752996963866No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11670.1150006356076261No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11510.11342393451960382No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11260.11096033906956898No Hit
CTGGTATCCCAACCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11180.11017198852555785No Hit
CTGATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGT10910.1075113054395202No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10710.10554042907949233No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10680.10524479762548816No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10630.10475207853548119No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10590.10435790326347562No Hit
GATTATACTGGTGACAGTAATACACTGCAAAATCTTCAGACTGTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCC10270.10120450108743104No Hit
GTCTCTGGTGCTTCCATCAGCGGCGCTCATTACTACTGGAGTTGGATCCG10260.10110595726942964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.036.9490661
TTCGGGG6100.031.59603770
TATCAAC27400.031.4006292
ATCAACG28200.030.49633
TCAACGC28100.030.3579714
CAACGCA28550.029.8794785
AACGCAG28550.029.759486
TAGTCGG605.068768E-429.08257351
AGAGTAC31500.027.18999911
CGCAGAG31700.026.8023058
GTGGTAT6200.026.5705381
ACGCAGA32350.026.3696777
GCAGAGT33300.025.720279
CAGAGTA34050.024.95251310
GTATACT1101.2250239E-624.9159264
CAAACGA13700.024.61954170
GTTATAC851.5122992E-424.1854883
AGTACGG26300.023.5777613
GAGTACG27400.022.88127712
GTACCGG750.002054304122.8801841
Produced by FastQC (version 0.11.5)