FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247426
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGTAGTCACTTCTGGAGCTGGATCCGGCAGCC48880.21749325673014372No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47950.21335518944783946No Hit
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT45380.20191988523760068No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC40170.17873780938727238No Hit
GTGTATTACTGTGCGAGAGACGCCCTTACCCCAAATTGTGGCGGTGCTTG38100.1695272725331112No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33950.15106170347766734No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT33750.1501717965352363No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33140.14745758036082168No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32450.1443874014094346No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31010.1379800714239312No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC30170.1342424622657209No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT29590.13166173213267088No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27850.12391954173352092No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27790.12365256965079163No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27630.12294064409684678No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGCTTCTTCCTTCTCCTGGTGGCAGCTCCCACATGGGTC27120.12067138139364766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27050.12035991396379682No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26780.11915853959151491No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26380.11737872570665286No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT25840.11497597696208907No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG24790.11030396551432615No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA24210.10772323538127619No Hit
GTAATACACGGCCGTGTCCGCTGCGGTCACAGAGCTCAGCTTCAGGGAGA23660.10527599128959085No Hit
TGCTTAGAGGGTGCTTTTGATCTCTGGGGCCAAGGGACAAAGGTCACCGT23480.10447507504140292No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23330.10380764483457965No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23150.10300672858639172No Hit
CTCCTGCAGGGCCAGTCAGAGTGTAAGCAGCAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT22720.10109342866016499No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22490.10007003567636932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)