FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247426
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47560.21161987091009893No Hit
GTCTCTGGTGGCTCCATCAGTAGTCACTTCTGGAGCTGGATCCGGCAGCC42660.1898171508205387No Hit
GTATAATAACTGGCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT42480.18901623457235076No Hit
GTAATACACGGCCGTGTCCGCTGCGGTCACAGAGCTCAGCTTCAGGGAGA39240.1745997421049681No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39010.1735763491211724No Hit
GTGTATTACTGTGCGAGAGACGCCCTTACCCCAAATTGTGGCGGTGCTTG34790.15479931263587765No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT34500.15350894756935268No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT33940.1510172081305458No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33620.14959335702265614No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32100.1428300642601803No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30220.13446493900132864No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29890.13299659254631743No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT29070.12934797408235021No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27220.12111633486486317No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC26980.12004844653394593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26850.11947000702136577No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26710.11884707216166407No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25520.11355212585419942No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGG24050.10701130982733134No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23940.10652186100899429No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23830.10603241219065722No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22560.10038150310622018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44600.037.948751
GGTATCA20100.037.21471
TATCAAC49400.033.817882
ATCAACG51400.032.5472873
TCAACGC51300.032.541464
CAACGCA51550.032.450415
AACGCAG53450.031.6832716
GTGGTAT10550.029.0795961
CGCAGAG59100.028.5378488
AGAGTAC60050.027.74184411
ACGCAGA61900.027.4137787
GCAGAGT62800.026.8564789
CAGAGTA62700.026.73402610
AGTACGG46000.024.31803513
GAGTACG46950.023.89928812
TACACCC16300.023.4394825
GCGGTCA18900.022.2257123
TACGGGT5100.021.59694915
CGTACAC16650.021.29293815
GTATTAC16950.021.1205523
Produced by FastQC (version 0.11.5)