FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281320
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.1297454855680364No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT2930.10415185553817716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGC150.001796025273.86629566
AAGAGCG350.001464664238.75705327
GGTATCA5650.038.325291
GTATCAA12400.036.8354531
CTTATAC1750.034.800731
GTCGCTT400.002652443534.34076337
GTCATAT701.1298634E-633.8340451
TATACAC1600.033.8340453
TATCAAC13650.033.4622422
GTGGTAT2050.031.3583811
TCCGAGC1850.031.08521713
TGCCGTC901.768476E-731.0746245
ATCAACG15000.030.6761993
TCAACGC15100.030.4730474
CGAGACA1900.030.27794823
GAACGGG450.00502143730.14437327
CCGTCTT1051.9483196E-830.1030547
TACTCCT450.005078591430.0747055
GCCGTCT952.7858732E-729.50414746
ATCTCCG1950.029.49110210
Produced by FastQC (version 0.11.5)