FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855684
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC75790.8857241692026495No Hit
GTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT70600.8250709374021251No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52900.6182188751922438No Hit
GATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA39590.462670799033288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35010.40914636711683283No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34050.3979272722173139No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32180.37607341027762586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31970.37361923326835605No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC30670.35842670892525746No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29600.34592209273516855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29330.34276672229467886No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG28700.33540419126686954No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28350.33131389625141994No Hit
GTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG28240.33002837496085No Hit
GCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC27330.319393607920681No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25690.30022765413400276No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25100.29333258539367335No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT22730.26563544486048585No Hit
GTCCCTGATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGT22240.2599090318388564No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22220.2596753006951164No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21970.2567536613983667No Hit
GTCTCCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTT21670.253247694242267No Hit
AGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAGCCCCTAAGTCCCTGATCTATG20240.23653591746485852No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT19830.23174442901818895No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT19400.22671920942777943No Hit
CAGTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT19210.2244987635622496No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19010.2221614521248498No Hit
ATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA18380.21479892109704052No Hit
GTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGAC18260.21339653423460064No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18220.21292907194712068No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGC18160.21222787851590072No Hit
GTGGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAA17930.209539970362891No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC17360.20287863276630158No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16760.1958666984541022No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16360.19119207557930262No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15980.18675118384824305No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG15800.18464760355458323No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15670.18312835112027337No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15510.18125850197035354No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC15470.18079103968287358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15360.1795055183923037No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15350.17938865282043373No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15120.17670074466742397No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15060.17599955123620403No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14820.17319477751132425No Hit
GATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAAT14820.17319477751132425No Hit
GTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14450.16887075135213467No Hit
CACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTACCCTTTCACTTT13830.16162508589619534No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13700.16010583346188545No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13660.1596383711744055No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13650.15952150560253553No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13460.15730105973700573No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTAC13360.15613240401830583No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13300.1554312105870859No Hit
GCCTGAAGACTTTGCAACTTATTACTGCCAACAGTATAATAGTTACCCTT13260.15496374829960594No Hit
CCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCG12740.14888673856236648No Hit
ATGCAGCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATTGCTAATGCC12620.1474843516999266No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12270.14339405668447697No Hit
TCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCATCACTTGTCGGGC11910.13918689609715737No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11870.1387194338096774No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11650.13614839122853764No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11490.1342785420786178No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11350.13264242407243795No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11340.13252555850056796No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11330.13240869292869797No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11280.13182436506934805No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10960.12808466676950836No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10910.12750033891015844No Hit
GTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG10800.12621481761958853No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10790.12609795204771854No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10360.121072732457309No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10040.11733303415746935No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGACT9970.11651497515437943No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTG9930.11604751286689947No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9860.11522945386380953No Hit
ACTTATTACTGCCAACAGTATAATAGTTACCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA9830.11487885714819956No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA9730.11371020142949967No Hit
GCCCCTAAGTCCCTGATCTATGCTGCATCCACTTTACAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGAT9710.11347647028575969No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC9550.11160662113583987No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9490.11090542770461993No Hit
GAATCAGACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTT9370.10950304084218006No Hit
GTATCAACGCAGAGTACGGGAATCAGACCCAGTCAGGACACAGCATGGAC9360.10938617527031005No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9130.10669826711730032No Hit
GAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAAG9080.10611393925795036No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8910.10412722453616054No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8910.10412722453616054No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8890.10389349339242056No Hit
ACCCAGTCTCCATCCTCACTGTCTGCGTCTGTGGGAGACAGAGTCACCAT8870.10365976224868058No Hit
CTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAATTGCTAA8820.10307543438933064No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8740.10214050981437073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)