EGAF00005758250 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005758250
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20657
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	106	0.5131432444207775	No Hit
ATATTATAGTGTACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT	91	0.4405286343612335	No Hit
ATAGTGTACCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA	63	0.3049813622500847	No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTA	58	0.2807764922302367	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	58	0.2807764922302367	No Hit
CTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC	55	0.2662535702183279	No Hit
CTCCTAAGCTGCTCATTACCTGGGCATCTACCCGGGAATCCGGGGTCCCT	47	0.22752577818657113	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	46	0.22268480418260153	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	45	0.21784383017863196	No Hit
GTACACTATAATATTGGTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT	36	0.17427506414290556	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	34	0.16459311613496636	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	34	0.16459311613496636	No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAACGGTCAGGGACC	32	0.15491116812702715	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	32	0.15491116812702715	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	32	0.15491116812702715	No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG	32	0.15491116812702715	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	32	0.15491116812702715	No Hit
GTCCCAGACCCGCTGCCACTGAAACGGTCAGGGACCCCGGATTCCCGGGT	30	0.14522922011908798	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	30	0.14522922011908798	No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT	29	0.14038824611511835	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	29	0.14038824611511835	No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA	28	0.13554727211114878	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	28	0.13554727211114878	No Hit
GTCCCTGACCGTTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC	28	0.13554727211114878	No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC	26	0.12586532410320958	No Hit
GGTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA	26	0.12586532410320958	No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAAACGGTCAGGGACCCCG	25	0.12102435009923997	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	25	0.12102435009923997	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	25	0.12102435009923997	No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCCTCAACTGCAAGTCCAGCCGAAATATTCTATC	25	0.12102435009923997	No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC	25	0.12102435009923997	No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG	24	0.11618337609527038	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG	24	0.11618337609527038	No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA	24	0.11618337609527038	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	23	0.11134240209130077	No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTGTTATTGTCGGAGCTG	23	0.11134240209130077	No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG	22	0.10650142808733118	No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGTAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA	22	0.10650142808733118	No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT	21	0.10166045408336156	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	21	0.10166045408336156	No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC	21	0.10166045408336156	No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA	21	0.10166045408336156	No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTACCTGGGCATCTACCCGGGAA	21	0.10166045408336156	No Hit
CAATAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA	21	0.10166045408336156	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAACAC	20	0.0065945005	53.00954	47
ACACAAA	20	0.0065945005	53.00954	50
AACACAA	20	0.0065945005	53.00954	49
GAGAAAC	20	0.0067287437	52.74114	45
GAAGTCA	25	0.0070474013	52.081875	69
GATGACC	20	0.0077262153	50.935818	29
GGGTCCC	35	0.0012137989	40.08225	43
GGGGTCC	35	0.0012290234	39.981285	42
TCTTCAT	90	1.235245E-4	36.56793	70
GGTCCCT	40	0.0023099857	35.16076	44
CGGGGTC	40	0.002368232	34.983627	41
TCGTGAT	40	0.0028431492	33.70995	25
CGTGATG	40	0.0028431492	33.70995	26
CATCTGT	55	2.3078735E-4	33.05212	64
ACCATCT	55	2.3802073E-4	32.87997	62
GGCAGCG	45	0.0034896194	32.317627	63
ACTGTGG	55	2.729302E-4	32.127	51
GTCCCTG	45	0.0041143736	31.254007	45
TATCAAC	65	2.6370664E-5	31.041534	2
AGTACTT	55	3.65819E-4	30.571207	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content