FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024389
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38350.1894398754389596No Hit
GTATAATAGCTGGCCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT36570.18064709895183187No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAAATATA31260.1544169623525913No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA29270.1445868358304654No Hit
CTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC27220.13446032358405427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24150.11929525402479464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23900.11806031350693963No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATA22820.11272537046980596No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22670.11198440615909294No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21750.10743982505338648No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGCACCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT20610.10180849629196762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.039.644591
GTATCAA39850.035.6649741
GTGGTAT7000.033.2684821
TGGTATC7350.032.616162
TATCAAC43950.032.0261762
ATCAACG45650.030.6092193
TCGTATG1600.030.22139440
TCAACGC46600.030.0587044
CAACGCA47900.029.2429145
AACGCAG49200.028.3324176
CGTATGC1705.456968E-1226.43741
CGCAGAG54300.025.7331648
AGAGTAC54800.025.6227311
ACGCAGA55700.025.086377
GCGTTGT13250.024.61566262
TCTGGAG23450.024.44956870
TACGGTA10100.024.42282770
CGTTGTC13700.024.11954763
GCAGAGT58650.023.9413539
AATATAG14650.022.73426645
Produced by FastQC (version 0.11.5)