FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024389
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39560.1954169875453779No Hit
GTATAATAGCTGGCCTCAGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT36200.17881938698540648No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACTA28670.14162297858761336No Hit
CTATTATACTGCTGACAGTAATAAACTGCAATATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC28110.1388567118276181No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTACTCATTTGCAAATATA26490.1308542972719176No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24250.11978923023193665No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23960.11835669923122483No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23760.1173687468169408No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22700.11213259902123555No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATA21970.10852657270909889No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21790.10763741553624327No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20560.10156150818839661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8600.037.8217431
GGTATCA20050.035.6901441
GTATCAA41100.035.4882161
TGGTATC9200.035.3306432
TATCAAC47100.030.510142
ATCAACG47500.030.384513
TCAACGC47750.030.2231864
CAACGCA49700.029.2437955
AACGCAG50550.028.8197126
AGAGTAC55650.026.24000411
CGCAGAG56000.025.9538698
GCGTTGT10600.025.76800762
ACGCAGA57250.025.3871927
GCAGAGT58600.025.0357679
TGGCGTT12100.023.37168560
CGATTCA9700.023.27394370
CGTTGTC11500.023.18733463
TAGTGAG12000.022.64086349
TTCGGGG11500.022.60546970
GTGTATA4250.022.5572171
Produced by FastQC (version 0.11.5)