FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100495
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36840.33475844960676787No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT24360.2213549357334654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23280.21154117010981424No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA20460.18591633764805837No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20040.18209987323886068No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATCGGAGGCCAGTCGCTACGCTG18770.17055961181104867No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18630.16928745700798278No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18460.16774269760425992No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18200.1653801243985661No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18160.16501665159769013No Hit
GTGTTAGGAAGTCCTTAGCCTGGTATCAACAGAGGCCTGGCCAGCCTCCC17890.16256321019177733No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTATTGTCAGCAGCGTAGCGAC15970.1451165157497308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15790.14348088814578894No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC15780.14339001994556994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15700.142663074343818No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTGTT14760.13412146352323273No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAAGTCCTTAGCCTGGTATCAACAGAGGCCTGGCCAGCCTCCCAGGCT14620.13284930872016684No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14170.12876023971031217No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13960.12685200750571335No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTATTGTCAGCAGCGTAGCGACTGG13590.12348988409761061No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13550.12312641129673466No Hit
GGTATCAACAGAGGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAG13500.12267207029563967No Hit
GTATCAACAGAGGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGC12620.11467566867636836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC11730.10658839885687803No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11520.10468016665227921No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11430.10386235285030827No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11200.10177238424527145No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11120.1010454386435195No Hit
GCGTAGCGACTGGCCTCCGATCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT11090.10077283404286252No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11090.10077283404286252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT200.007292334551.86913338
TCCGATA200.007504466551.4943282
AGTATCG901.2369128E-1038.3145934
ATCGCGT400.0025769834.55253237
ATCAACG33650.034.4825863
TCAACGC34050.034.07754
CAACGCA34600.033.535815
AACGCAG34750.033.4898386
AGAGTAC38100.030.17792111
CGCAGAG38850.029.9487138
ACGCAGA39300.029.605797
ACCGATC2350.029.23923327
GCAGAGT40350.028.8353799
CAGAGTA40350.028.58019810
ACGAGAC2550.028.27396822
TTAACGC500.0070943128.0930759
TGTTAGA5100.028.03040370
TTAGACC500.00792397327.4636444
GTAGTTT2853.8198777E-1127.3598270
GTATCAA46550.027.2866481
Produced by FastQC (version 0.11.5)