FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100495
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37090.33703015461224267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22740.20663428729798863No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT22720.20645255089755066No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21520.19554836687127156No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21460.19500315766995763No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACACCA19850.18037337743469986No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGATCGGAGGCCAGTCGCTACGCTG18570.1687422478066688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18460.16774269760425992No Hit
GTGTTAGGAAGTCCTTAGCCTGGTATCAACAGAGGCCTGGCCAGCCTCCC18390.16710662020272696No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17290.1571111181786378No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT15960.1450256475495118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15930.14475304294885483No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15780.14339001994556994No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC14760.13412146352323273No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTATTGTCAGCAGCGTAGCGAC14720.13375799072235675No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGCGGCCCTGTT14190.12894197611075017No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAAGTCCTTAGCCTGGTATCAACAGAGGCCTGGCCAGCCTCCCAGGCT14160.12866937151009317No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTATTGTCAGCAGCGTAGCGACTGG13770.12512551170155248No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13530.12294467489629667No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13450.12221772929454473No Hit
GGTATCAACAGAGGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAG13340.12121817909213581No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12740.11576608707899627No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12530.11385785487439742No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12290.1116770180691416No Hit
TCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT12000.10904184026279083No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11920.1083148946610389No Hit
GTATCAACAGAGGCCTGGCCAGCCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCGCTGGCATCCCAGC11730.10658839885687803No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11440.10395322105052726No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC11190.10168151604505245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)