FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994255
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13120.1319580992803657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31150.036.0865941
TCAACGC35900.030.712854
ATCAACG36050.030.5881353
TATCAAC36500.030.4109022
CAACGCA36900.029.9733185
AACGCAG37450.029.4416926
GGTATCA14800.029.408451
ACGCAGA40500.027.055387
CGCAGAG40350.026.9013718
AGAGTAC40050.026.6753911
CAGAGTA41100.026.07721510
GCAGAGT42500.025.6210489
ACGCTAA1400.001943877523.09612570
AGTACGG32850.022.51526513
GTGGTAT7050.022.3613931
GAGTACG33550.022.14756212
TAACGGC2501.8189894E-1220.69399636
GAGTACT16100.020.20488712
CGAACTA3600.020.03291129
CGCGGTA2850.019.49115443
Produced by FastQC (version 0.11.5)