FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212822
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTCACCTCTCAGGCTGTTCATTTGCAGAAACA86440.3906324141752025No Hit
GTATATTACTGTGCGACAGACCCCGTTGTAGGGATCTGTAGTGGCGGCGG71400.32266490481385307No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAAGCACTCAGGCTGCTATCCCATGTTGCGCAGTAATAAT64810.2928839283051235No Hit
ACATGGGATAGCAGCCTGAGTGCTTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCC58550.26459426017998733No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG55490.2507657642593937No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55230.2495907940177746No Hit
ATCCCATGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG52560.2375247534596095No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGGCCAGGGTGG50570.22853171199490965No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAAAGCACTCAGGCTGCTATCCCATGTTGCG46690.21099754069690196No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43760.19775652989711778No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43410.19617483918724596No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC43160.19504506010876607No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG41240.18636835678604063No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT40900.184831857239308No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40100.1812165641881724No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39480.1784147120735423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39360.17787241811587196No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38800.17534171298007703No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC38470.17385040459648357No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA38200.17263024319172532No Hit
CTCCTGTGCAGCGTCTGGATTCAGTTTTGGTGACTATGGCATGCACTGGGTCCGCCAGGCCCCAGGCAAGGGG37690.17032549387162638No Hit
GATTATTACTGCGCAACATGGGATAGCAGCCTGAGTGCTTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT36130.16327567242191193No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG35200.15907289424996676No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC33910.15324323420501063No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC33820.15283651373675786No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG33570.15170673465827797No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33500.15139039651630362No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG32440.14660013322354892No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAATAATAAGCGACCCTCAAGGATTCCTGACCGATTCTCTGG32000.14461172204542433No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31480.14226178156218622No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30890.13959550293697368No Hit
GGGCTGGAGTGGGTGGCCTTTGTACGGACTGATGGAAGTGATAAATACTA30880.13955031177383448No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30580.13819457687965864No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29780.13457928382852302No Hit
GGACAGAAGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACTTTGAGAATAATTTTGTATCCTGGTACCAGCAG29510.13335912242376477No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG29430.1329975931186512No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCATCACTGAACACAGA29300.13241010799784167No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC29230.1320937698558673No Hit
GTCTGGAGTCCGGTGATGGCCAGGGTGGCTGACGTGCCAGACTTGGAGCC28830.13028612333029949No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG28500.12879481494670605No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACAAAATTATTCTCAAAGTTGGA28140.12716793307369503No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27730.12531509538498803No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27550.12450165444848253No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC27270.12323630188058506No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26320.11894314138236152No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT26190.11835565626155198No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC26160.11822008277213442No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25900.11704511253051533No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA25590.11564418647320028No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC25480.11514708367866913No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25200.11388173111077167No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25160.11370096645821488No Hit
CCCTCAGTGTCTGCGGCCCCAGGACAGAAGGTCACCATCTCCTGCTCTGG24980.11288752552170939No Hit
CTCCAGCCCCTTGCCTGGGGCCTGGCGGACCCAGTGCATGCCATAGTCAC24110.10895589432859941No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24090.10886551200232102No Hit
ACTCCAGACTGGGGACGAGGCCGATTATTACTGCGCAACATGGGATAGCA24040.10863955618662503No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23880.10791649757639793No Hit
GTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATTCTAAGAACATGG23240.10502426313548945No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23070.10425601336212312No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23000.10393967522014874No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23000.10393967522014874No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG22850.10326180777306083No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22800.10303585195736485No Hit
CTCCTGCTCTGGAAGCAGCTCCAACTTTGAGAATAATTTTGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCC22710.1026291314891121No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT22710.1026291314891121No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22590.10208683753144174No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22510.10172530822632819No Hit
GTTTGGGGGCTGTTCCTGGGAGCTGCTGGTACCAGGATACAAAATTATTC22510.10172530822632819No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT22500.101680117063189No Hit
GGATAGCAGCCTGAGTGCTTTATTCGGCGGAGGGACCAAGCTGACCGTCC22480.10158973473691062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.047.1914861
GGTATCA14200.044.0821881
TGGTATC4850.041.1297952
GTATCAA36250.040.323761
TATCAAC39500.036.8310052
TCGTATG1051.6370905E-1136.21821640
CGTATGC1151.8189894E-1236.0963841
ATCAACG41450.035.09833
TATACAC2650.035.0419433
TATCGCA400.002482617734.81791352
TCAACGC41950.034.680754
ACGAGAC2400.034.39224222
CAACGCA42350.034.1923075
AACGCAG43600.033.054996
TTATACA2550.031.0211522
AATGGTA9250.030.69157270
AGAGTAC47400.029.89232411
CTTATAC2650.029.851221
GAGCGAT2100.029.53178632
CAGAGTA48100.029.52946710
Produced by FastQC (version 0.11.5)