FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314843
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49640.2144421889519073No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT36620.15819647379973503No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA35980.1554317074635299No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33710.1456254268648025No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31990.1381951173362513No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29850.12895042989956554No Hit
CTATGATACTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC29620.12795684199749183No Hit
GTCTATTACTGTGCGAGGGGGGGATATTGTACTACTACCAACTGCTATATGGGTCTTCGGAGTGACTACTGGTA28970.12514887618728354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28490.12307530143512974No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28150.12160651931902076No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28130.12152012037101435No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG27900.12052653246894066No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA27400.11836655876878042No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27360.11819376087276762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27200.11750256928871633No Hit
GTATCATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT27160.11732977139270351No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACAGTATCATAGTTATTGGACGTTCGG26530.11460820453050163No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA25790.1114114434542645No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTA25010.10804188448201456No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24950.10778268763799533No Hit
GTGCAGCCACAGTTCGTCTGATATCCACCTTGGTCCCTTGGCCGAACGTC24360.10523391867180625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24020.1037651365556973No Hit
GTCTCTGGTGGCTTCATCAATAATTACTACTGGACTTGGATCCGGCAGCC23940.10341954076367166No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23760.10264195023161399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)