FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314843
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50120.21651576370406111No Hit
CTATGATACTGTTGGCAGAAATAAGTTGCAAAATCATCAGGCTGCAGGCTGGTGATGGTGAGAGTGAATTCTGTC32990.14251506473657177No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGT32790.1416510752565077No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGAGTTCAGCTTCAGGGAGA31730.137071931012168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30640.1323631883458187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29770.1286048341075399No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29420.12709285251742775No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29370.12687685514741173No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28660.1238096924931842No Hit
GTATCATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGATATCAGACGAACTGTGGCTGCACCATCTGTCTT27450.11858255613879645No Hit
GTCTATTACTGTGCGAGGGGGGGATATTGTACTACTACCAACTGCTATATGGGTCTTCGGAGTGACTACTGGTA27330.118064162450758No Hit
GTGCAGCCACAGTTCGTCTGATATCCACCTTGGTCCCTTGGCCGAACGTC27130.11720017297069392No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26260.11344181873241511No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25690.11097944871423246No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25440.10989946186415235No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24690.106659501313912No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24370.10527711814580945No Hit
CACCATCACCAGCCTGCAGCCTGATGATTTTGCAACTTATTTCTGCCAACAGTATCATAGTTATTGGACGTTCGG23900.10324674286765884No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA23740.10255555128360758No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23720.10246915233560117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG200.00738540251.704871
GTATCAA45450.039.361681
GTGGTAT9100.037.879021
TATCAAC51600.034.646332
ATCAACG52950.033.4832423
TCAACGC52900.033.3146174
CAACGCA53700.032.946515
TACTGAA16250.032.5104270
AACGCAG55150.032.3299336
AGAGTAC60250.029.93542511
CGCAGAG60400.029.5191618
ACGCAGA61300.029.1425447
GCAGAGT63450.028.3171849
GAGTACG55000.023.90627112
AGTACGG54800.023.867913
TGTTAGA10700.023.74918270
CGAACTA1752.4374458E-1023.63855629
TGGATTG12850.023.41845570
AATAGCG1854.0199666E-1022.71260362
CTACTGA16250.022.41777669
Produced by FastQC (version 0.11.5)