FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570992
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39600.2520700296373247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49200.042.862541
TATCAAC55750.037.6788442
TCAACGC57900.035.9664344
CAACGCA58000.035.9044235
ATCAACG58750.035.6237953
AACGCAG58700.035.5344166
GGTATCA20800.033.850241
GTGGTAT7650.033.5087051
AGAGTAC64350.032.30732711
ACGCAGA65200.032.1489567
CGCAGAG67050.031.3128388
GCAGAGT68200.030.9850649
CAGAGTA67400.030.94664610
AGTACGG49150.025.90702813
GAGTACG50600.025.29956412
TGGTATC10200.025.1147252
GAGTACT32150.024.8466112
AGTACTT32350.024.79852513
GTACTTT33200.023.95797514
GTACGGG53550.023.14085614
Produced by FastQC (version 0.11.5)