FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427123
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG34790.24377716566827104No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG33020.2313745906975082No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT31480.22058364976249417No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG30330.21252547958374995No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29820.20895185628708948No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA26320.184426990525694No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG25000.17517761258139627No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24910.17454697317610327No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24350.17062299465427996No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23000.16116340357488457No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22980.16102326148481946No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT21590.15128338622529383No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21370.14974182323457755No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC20600.14434635276707053No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19740.1383202428942705No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19310.13530718795787047No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18530.12984164644533092No Hit
GCCCTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTA18530.12984164644533092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18440.12921100704003788No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18380.12879058076984254No Hit
ATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCTCAGCTTTGACTACTGGGGCCAGGGAAG18130.12703880464402859No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18050.12647823628376811No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17890.12535709956324717No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT17610.12339511030233553No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17190.12045212641096809No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAA17070.11961127387057736No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16370.11470630071829828No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16040.11239395623222385No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15940.11169324578189828No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15430.10811962248523778No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14830.10391535978328428No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGATTATGAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC14700.10300443619786102No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA14610.10237379679256797No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCAC14590.10223365470250288No Hit
CAGTAACACTTTGCTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA14570.10209351261243776No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14500.10160301529720983No Hit
ATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACAGCG14380.10076216275681914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)