FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427123
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTG33090.2318650880127361No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCAAAGTGTTACTGCCTGCATATGAGCTG29280.20516801985533134No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29150.20425709626990807No Hit
TCTCTGGGCTGCAGGCTGAGGATGAGGCTCATTATTACTGCAGCTCATAT29000.20320603059441966No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCACCATCTCCTG28330.19851127057723827No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG26860.18821082695745214No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT25120.17601846512178698No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24580.17223462869002884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24010.16824057912317297No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23290.16319546388082876No Hit
GTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAACA22590.15829049072854967No Hit
GTGTTACTGCCTGCATATGAGCTGCAGTAATAATGAGCCTCATCCTCAGC21530.15086295995509846No Hit
CTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCT20520.14378578440681006No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19840.13902095334459608No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19820.13888081125453097No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18960.13285470138173092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18760.13145328048107977No Hit
GCCCTATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTA18330.12844022554467974No Hit
ATATTACTGTGCGAAAGAGACGGGCTACAATCTTTTGACTGGCTATCTCAGCTTTGACTACTGGGGCCAGGGAAG17970.12591766792350764No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17760.12444617597782391No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17540.12290461298710763No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17410.12199368940168437No Hit
CTCCTGCACTGGAACCACCAGTGACGTTGGTGATTATGAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC17060.11954120282554481No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGGTGGCTTTTT16900.1184200661050239No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16880.11827992401495876No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16700.1170186452043727No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16520.11575736639378666No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCGGGACAGTCAGTCAC15870.11120274846667035No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15770.11050203801634478No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15090.10573720695413079No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14950.10475621232367498No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14830.10391535978328428No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14740.10328472037799123No Hit
CAGTAATATAGGGCCGTGTCGTCGGCTCTCAGGCTGGTCATTTGCAGAAA14680.1028642941077959No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)