FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859299
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29210.339928243835964No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACTGCAGCCTCAGGGAGA28220.3284072249589491No Hit
GTCTCTGGTGCTTCCATCAACAGTAGTGATTACTACTGGGGCTGGATCCG27770.3231703981966696No Hit
GTGTATTACTGTGTGAAACAGGACTACGGTGGCAGCTTGGGCGACAGGTT27730.322704902484467No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25510.29686989045722156No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCAGCATATCCGTTGGCACGGCGAAGAACCACTTCTCCCTGAGG25430.2959388990328163No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24170.28127578409843373No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23400.2723149916385333No Hit
CGCCTGCACTGTCTCTGGTGCTTCCATCAACAGTAGTGATTACTACTGGG22000.25602264171144157No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20740.24135952677705896No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20460.23810105679164065No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20350.23682094358308342No Hit
GTAGTAGGTGGTCCCAGAAGAATAGATACTGCCAATCCACTCCAGACCCTTCCCGGGGGGCTGGCGGATCCAGCC20310.2363554478708808No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20070.23356247359766508No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA18340.2134297840449017No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACTGCAGCCTCAGGGA17330.20167601731178553No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17050.1984175473263672No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16360.190387746290872No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16020.1864310327371497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15410.17933222312605973No Hit
GGTCTGGAGTGGATTGGCAGTATCTATTCTTCTGGGACCACCTACTACAG15310.17816848384555317No Hit
GTAGGAGACAGCGTCACCATCACTTGTCGGGCGAGTCAGGGTATTGTCAACTGGTTAAGCTGGTATCAGCAGAAA14820.17246616137107107No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14690.17095330030641256No Hit
GTATTACTGTGTGAAACAGGACTACGGTGGCAGCTTGGGCGACAGGTTCGACCCCTGGGGCCAGGGAACCCTGGT14570.1695568131698047No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14410.16769483032099422No Hit
CCTGTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTCCTGTCCC14370.16722933460879158No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAACGTC14350.16699658675269027No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14000.16292349927091734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13950.16234162963066406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13900.16175975999041078No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13740.1598977771416003No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13220.15384633288296623No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13150.15303171538661164No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13020.1515188543219531No Hit
GGGTATTGTCAACTGGTTAAGCTGGTATCAGCAGAAACCGGGCAAAGCCCCCAAACTCCTGATCTATCGTGCAT12860.14965687147314263No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12420.1445364186389138No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12390.14418729685476184No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12380.1440709229267112No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12160.14151069650959677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12130.14116157472544483No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA11860.13801947866807712No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC11820.13755398295587448No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11820.13755398295587448No Hit
ACTACAGCCCGTCCCTCAAGAGTCGAGTCAGCATATCCGTTGGCACGGCG11730.1365066176034186No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT11660.135692000107064No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC11400.13266627797774697No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC11320.13173528655334174No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11160.12987330370453126No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11130.1295241819203793No Hit
GGGTTCCCTGGCCCCAGGGGTCGAACCTGTCGCCCAAGCTGCCACCGTAGTCCTGTTTCACACAGTAATACACGG10950.12742945121546748No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10780.12545109443860636No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT10740.12498559872640372No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA10720.12475285087030243No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA10710.12463647694225177No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG10640.12382185944589717No Hit
GTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGGTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC10570.12300724194954259No Hit
GGGCTGGTGAAGCCTTCGGCGACCCTGTCCCTCGCCTGCACTGTCTCTGGTGCTTCCATCAACAGTAGTGATTA10570.12300724194954259No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG10310.11998151982022555No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT10300.1198651458921749No Hit
ACCCTGTCCCTCGCCTGCACTGTCTCTGGTGCTTCCATCAACAGTAGTGATTACTACTGGGGCTGGATCCGCCAG10290.11974877196412426No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10230.11905052839582032No Hit
GGCTAACAGTTTCCCTCCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGGCTGCACCAT10200.11870140661166834No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG10170.11835228482751639No Hit
AGTCAGGGTATTGTCAACTGGTTAAGCTGGTATCAGCAGAAACCGGGCAA10130.11788678911531378No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10100.11753766733116179No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG10070.11718854554700983No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGGT10050.11695579769090853No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9960.11590843233845263No Hit
CATCTAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTG9950.11579205841040197No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9900.1152101887701487No Hit
GATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGGCTGGTGAAGCCTTCGGCGACCCTGTCCCTCG9820.11427919734574345No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9800.11404644948964215No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9700.11288271020913558No Hit
ACCTAGATGGGACCCCATTCTGCAAAGTAGATGCACGATAGATCAGGAGTTTGGGGGCTTTGCCCGGTTTCTG9660.11241721449693295No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCCGGGAAGGGTCTGGAGTGGATTGGCAGTATCTATTCTTCTGGGACCAC9660.11241721449693295No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9640.11218446664083166No Hit
GTCCCTCGCCTGCACTGTCTCTGGTGCTTCCATCAACAGTAGTGATTACTACTGGGGCTGGATCCGCCAGCCCCC9380.10915874451151461No Hit
GTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGGCTGGTGAAGCCTTCGGCGACCCTGTCCCTCGCCTGCA9280.10799500523100806No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACT9280.10799500523100806No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC9230.10741313559075479No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC9170.10671489202245085No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9160.1065985180944002No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCA9110.10601664845414692No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT9050.10531840488584299No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9010.10485290917364037No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG8980.10450378738948841No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8960.1042710395333871No Hit
GTCACAGACTGCAGCCTCAGGGAGAAGTGGTTCTTCGCCGTGCCAACGGA8890.10345642203703251No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8880.10334004810898187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC650.058.60299741
TCGTATG601.8189894E-1257.66768340
ACGTTAG200.006856591452.6826161
GTATCGC200.00738441651.70381535
GTTAGCT200.007430582351.62238331
CGAAATC200.0074959251.5082123
AGTACCC200.007502822751.496228
CTTATAC1550.050.960291
CGATCAG1600.049.4310829
ACCGATC1600.049.40804327
TATACAC1650.047.8717883
CCCACGA1700.046.4502719
ATCTCCG1700.046.44756710
CTCGTAT751.6370905E-1146.09089739
CCGATCA1800.045.83843228
GTATCAA14750.045.4023441
ACGAGAC1750.045.13122
TCTCCGA1750.045.120511
AACGGAT11000.044.8900270
ATCAGTA1800.043.9746231
Produced by FastQC (version 0.11.5)