FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1555924
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT753.9388397E-836.88770339
GTATCAA42200.033.1872521
TAAGGCG2550.030.9696429
GGTATCA20500.030.0662361
ATCAACG47900.029.381013
TCAACGC47750.029.3298844
CAACGCA48400.028.9359915
AACGCAG49150.028.4944466
TATCAAC49750.028.4261052
GACTAAG3200.027.8530426
ACGCAGA53050.026.3962657
AGAGTAC53050.026.13811111
CAGAGTA53500.025.98225610
ACGAGAC3300.024.90734922
CGCAGAG56250.024.8946118
GTGGTAT10950.024.7043481
GCAGAGT57450.024.6130039
TCGTATG1151.6498798E-624.07986640
GTCTAGT1902.910383E-1123.4288041
GAGTACG45300.023.12745312
Produced by FastQC (version 0.11.5)