FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724121
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10260.14168902711011006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA200.006588919653.2148264
CGTAAGC200.00726954151.90838640
TCGGTAA305.9447414E-446.55969250
TATTACG459.463549E-538.70168747
GTATCAA20550.037.739251
TACGTAT501.7330474E-434.9197750
TTACGTA501.7387913E-434.9001249
AATCGCA501.8015383E-434.69027343
GTGGTAT3700.033.3885351
ATCAACG23650.033.0369573
TCAACGC23900.032.684614
TATCAAC23950.032.491272
CAACGCA24050.032.480755
GGTATCA9400.032.1256141
AACGCAG24450.031.949376
ACGCAGA26600.029.3669977
CGCAGAG27650.028.3757068
AGAGTAC27200.028.34131211
GCAGAGT27700.028.3244889
TGGTATC3850.027.6024272
Produced by FastQC (version 0.11.5)