FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812727
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47730.5872820762691532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31680.38979878852308336No Hit
GATTTTACACTGAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATT31130.38303144844455767No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30680.37749453383485476No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28820.3546086201147495No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28510.3507943011613986No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27810.34218132287963854No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27760.3415661101452271No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26420.32507840886300077No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24540.30194641004913086No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24350.29960860165836745No Hit
GTCCCTGACAGGTTCAGCGGCAGTGGATCAGGCACAGATTTTACACTGAG22800.28053700689161304No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT22350.2750000922819102No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA21730.2673714543752084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21720.26724841182832615No Hit
CTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG21350.26269583759368154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20110.24743856178027798No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19170.23587256237334306No Hit
GGCCAAAAGTGTACGGAATTTGTAGAGCTTGCATGCAATAATAAACCCCA18460.22713654154470075No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC18290.22504481824770187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18140.2231991800444676No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17130.21077188280935666No Hit
GCAGTGGATCAGGCACAGATTTTACACTGAGGATCAGCAGAGTGGAGGCT16680.20523496819965376No Hit
GAATTTGTAGAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCCTCAGTGTAAAAT16520.20326628744953718No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15780.19416113898024798No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15460.19022377748001482No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA14990.18444077777654735No Hit
GTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAAT14660.1803803737294319No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACT14650.18025733118254963No Hit
GAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGC14580.1793960333543736No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACTCTCC14580.1793960333543736No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT14570.17927299080749132No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCGCTGAACCTGTCAGGGACCCCG14530.17878082061996217No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14470.17804256533866847No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14240.17521258676037588No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT14170.17435128893219987No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14160.1742282463853176No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14150.17410520383843528No Hit
GTTCAGCGGCAGTGGATCAGGCACAGATTTTACACTGAGGATCAGCAGAG14100.17348999110402386No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13780.1695526296037907No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGGCCAGGGGA13730.16893741686937927No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13660.16807611904120326No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13590.16721482121302725No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13420.16512309791602836No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA13340.1641387575409701No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13250.1630313746190295No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13090.16106269386891292No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAAT12920.15897097057191406No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12910.15884792802503178No Hit
TTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGGCCAGGGGA12870.15835575783750264No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12730.15663316218115061No Hit
GCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTA12640.15552577925921005No Hit
GGTCTAGTCAGAGTCTCCTCCATAGTAATGTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC12620.15527969416544546No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12510.15392622614974033No Hit
GATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGG12450.1531879708684466No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12180.14986582210262486No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGA12000.1476510562587437No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11650.1433445671178637No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11420.1405145885395711No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGT11400.14026850344580652No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11280.13879199288321908No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11030.13571592921116193No Hit
GTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC10930.13448550374233906No Hit
GTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGAGTTGGCTCCG10720.13190161025781105No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10680.1314094400702819No Hit
CCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGTCTCCTC10550.12980988696081217No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTC10520.12944075932016533No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10340.12722599347628416No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10330.12710295092940188No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10320.1269799083825196No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10260.12624165310122587No Hit
CCATAGTAATGTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC10040.12353471706981556No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9970.12267341924163958No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9500.11689041953817211No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9390.11553695152246696No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT9360.11516782388182008No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9360.11516782388182008No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9290.1143065260536441No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9000.11073829219405777No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8980.11049220710029323No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGA8930.10987699436588179No Hit
GGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAA8910.1096309092721172No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATACATTACTATGGAGGAGACTCTGA8790.10815439870952978No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8710.10717005833447149No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8600.10581659031876633No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8460.10409399466241431No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATACATTACTATGG8430.10372486702176745No Hit
ATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAG8410.10347878192800289No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG8410.10347878192800289No Hit
CAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCCTCAGTGTAAAATCTGTGCCTGATCCACTGCCG8410.10347878192800289No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8400.1033557393811206No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8350.10274052664670916No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT8350.10274052664670916No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT8290.10200227136541544No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8250.10151010117788631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG200.007431295751.6208888
TGGTATC1750.045.243652
CGTATGC851.8189894E-1244.81970241
GTGGTAT1900.043.4835971
GTTATTA754.8166094E-543.39419670
GTATCAA12200.042.0430531
GGTATCA4750.039.135241
TATGCCG1009.094947E-1238.129843
TCGTATG1009.094947E-1238.082640
TATCAAC14200.035.879072
TCAACGC14750.034.543334
ATCAACG15000.033.967613
CAACGCA15100.033.742665
ACGAGAC1253.6379788E-1233.04143522
ATGCCGT1056.0754246E-1033.0291944
AACGCAG15750.032.3501056
CACGAGA1400.031.95971921
TGCCGTC1109.949872E-1031.54350945
TATCTCG1101.0422809E-931.40906736
ATAGTAC450.004670283330.601453
Produced by FastQC (version 0.11.5)