FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812727
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50120.6166892449740196No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG30560.37601802327226735No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30230.37195761922515186No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29720.3656824493341552No Hit
GATTTTACACTGAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATT29610.3643289813184501No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29240.35977640708380554No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26800.3297540256445276No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26150.321756260097179No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23540.28964215536090226No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCGCTGAACCTGTCAGGGACCCCG23170.28508958112625765No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22680.27906049632902563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22340.27487704973502786No Hit
CTACAAATTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG20990.2582663059059192No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA20440.25149896582739345No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT20360.25051462545233516No Hit
GTCCCTGACAGGTTCAGCGGCAGTGGATCAGGCACAGATTTTACACTGAG20210.24866898724910086No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19640.24165556207681052No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18960.23328866888881505No Hit
GGCCAAAAGTGTACGGAATTTGTAGAGCTTGCATGCAATAATAAACCCCA18860.2320582434199922No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18610.22898217974793503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18190.223814392778879No Hit
GAATTTGTAGAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCCTCAGTGTAAAAT17520.2155705421377658No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16700.20548105329341831No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCC16140.19859067066801028No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15950.19625286227724684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15830.1947763517146594No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15760.1939150538864834No Hit
GCAGTGGATCAGGCACAGATTTTACACTGAGGATCAGCAGAGTGGAGGCT15640.19243854332389598No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15610.19206941568324912No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15130.18616337343289935No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14640.18013428863566733No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCT14610.17976516099502046No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14540.17890386316684448No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14400.17718126751049246No Hit
GTTCAGCGGCAGTGGATCAGGCACAGATTTTACACTGAGGATCAGCAGAG14270.17558171440102274No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA14090.17336694855714158No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13640.1678300339474387No Hit
GTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAAT13390.16475397027538152No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGGCCAGGGGA13250.1630313746190295No Hit
GAGGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGC13110.1613087789626775No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAAT13060.16069356622826605No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12970.15958618330632549No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACTCTCC12910.15884792802503178No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGA12730.15663316218115061No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12720.15651011963426834No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12690.15614099199362147No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12630.15540273671232774No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTCCACT12250.15072711993080087No Hit
GATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGG12230.15048103483703631No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12140.14937365191509572No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12120.14912756682133116No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCGGGAGAGCCGGCCTCCATCTCCT12070.1485123540869197No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12060.14838931154003743No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11730.14432890749292196No Hit
TTATTATTGCATGCAAGCTCTACAAATTCCGTACACTTTTGGCCAGGGGA11620.1429754394772168No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11090.13645418449245564No Hit
GCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTATTGCATGCAAGCTCTA11020.13559288666427963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10960.13485463138298592No Hit
CTGCTGATCCTCAGTGTAAAATCTGTGCCTGATCCACTGCCGCTGAACCT10850.13350116336728077No Hit
GTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGAGTTGGCTCCG10760.1323937804453402No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10740.1321476953515756No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10730.13202465280469333No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG10680.1314094400702819No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10670.13128639752339963No Hit
GGTCTAGTCAGAGTCTCCTCCATAGTAATGTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC10660.13116335497651732No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGA10300.12673382328875502No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10300.12673382328875502No Hit
GTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCC9990.12291950433540415No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9880.12156603631969899No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9740.11984344066334697No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGTCTC9700.11935127047581784No Hit
CACCTGCACTGTGTCTGGTGGCTCCATCACCGGTGGAGATTCCTACTGGA9640.11861301519452412No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGCGATATTGTGATGACTCAGT9590.11799780246011268No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9550.11750563227258352No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9490.1167673769912898No Hit
ATCCACTGCCGCTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCAAATAGATCAGGAGTTGTGGAGA9370.11529086642870238No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGG9140.11246088785040978No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9000.11073829219405777No Hit
CCCCGGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGTCTCCTC8990.11061524964717549No Hit
CAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCCTCAGTGTAAAATCTGTGCCTGATCCACTGCCG8990.11061524964717549No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8980.11049220710029323No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8950.11012307945964635No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8930.10987699436588179No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8830.10864656889705891No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8750.10766222852200062No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8590.10569354777188403No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG8560.10532442013123718No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT8530.10495529249059032No Hit
CCATAGTAATGTATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC8340.10261748409982688No Hit
GCTGATCCTCAGTGTAAAATCTGTGCCTGATCCACTGCCGCTGAACCTGT8330.1024944415529446No Hit
GCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTT8250.10151010117788631No Hit
GCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG8210.10101793099035715No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATACATTACTATGGAGGAGACTCTGA8150.10027967570906343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)