FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969586
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26450.039.766641
CTTATAC2150.036.6518251
ATCAACG30750.033.871523
TATCAAC31250.033.658482
CAACGCA31650.033.0165985
TCAACGC31850.032.8092734
AACGCAG32350.032.5139926
ACGAGAC2200.031.16126822
GGTATCA12500.030.1501081
CGTATGC1301.8553692E-1029.3384841
ACGCAGA36300.028.9759677
AGAGTAC36700.028.84686511
CGCAGAG36800.028.5822738
GCAGAGT37150.028.3129929
CAGAGTA37400.028.3069510
CGTCGTA500.00742389227.83261344
TCGTATG1253.969035E-927.71505440
GTGGTAT5200.027.0138661
CTCGTAT951.0221229E-525.5110639
AGTACGG29900.024.97994613
Produced by FastQC (version 0.11.5)