FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969586
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14240.14686680707023408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28200.037.440021
GGTATCA13250.034.4088551
TATCAAC32400.032.5564232
CAACGCA32300.032.4334455
TCAACGC32350.032.3833164
ATCAACG32700.032.0367053
AACGCAG32900.031.946016
CGTATAT553.4081237E-431.12139514
AGAGTAC35100.029.55202511
CGCAGAG35700.029.343038
CAGAGTA35550.029.27424410
GCAGAGT36350.029.1008639
ACGCAGA36650.028.6773197
CGGGATA756.485254E-527.39389217
GTGTACG658.986302E-426.3688451
GTGGTAT5500.026.177071
AGTACGG30600.024.38859713
TGGTATC5950.024.1748242
AGTTTCG1350.001499668224.14892470
GAGTACG31500.023.80045712
Produced by FastQC (version 0.11.5)