FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866047
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.036.8898961
GTATCAA19750.035.6316951
TCGTATG1003.7107384E-1034.5837140
TCAACGC22600.030.2286994
CAACGCA22800.029.9635355
ATCAACG22950.029.9155543
TATCAAC23400.029.4869562
AACGCAG23350.029.2577536
CGTATGC1103.5415724E-828.32377441
CGCAGAG25450.026.9768948
ACGCAGA25400.026.8948487
AGAGTAC25400.026.4878711
GTGGTAT4350.026.0420061
GGACCGT951.0913785E-525.2958476
CGAACTA2200.025.01210429
CAGAGTA26800.024.97753310
ATAGTAC1251.3263161E-724.7162323
TGGTATC5150.023.996342
GCAGAGT29200.023.3948359
ATTAGAG2155.456968E-1222.353183
Produced by FastQC (version 0.11.5)