FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866047
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12650.14606597563411686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC200.00754556551.42231412
GTATCAA18850.037.3275761
GGTATCA8650.036.1087571
ATCAACG22750.030.5949823
TATCAAC23000.030.4220452
TCAACGC22900.030.239614
CAACGCA23050.030.0428265
GTGGTAT4550.029.4198511
AACGCAG24000.028.8552956
ACGCAGA26100.026.664967
AGAGTAC25950.026.28912211
CAGAGTA26000.026.1082210
CGCAGAG26450.026.0528838
TGGTATC5550.024.7202632
GTATCGA700.001383861824.48681316
GCAGAGT28300.024.4709229
GTAGTTT3702.5465852E-1123.69434770
CGAAATT2056.548362E-1122.06696948
GCACTAT1550.002751659121.75411470
AGTACGG23400.021.2416113
Produced by FastQC (version 0.11.5)