FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972390
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47130.48468207200814484No Hit
CTGCTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGCC35810.3682678760579603No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33580.34533469081335677No Hit
GGCTAGCAGTTCCCCATACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT33140.34080975740186553No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTG31590.3248696510659303No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30150.31006077808286797No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28130.2892872201482944No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27760.2854821625068131No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAAC27710.28496796552823456No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26400.27149600468947643No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTGGCACCTGGTTAGCCTGGTATCAGAAGAAA24720.25421898620923705No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24640.2533962710435113No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23920.24599183455198018No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23720.24393504663766594No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22020.22645234936599512No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21970.22593815238741657No Hit
CTCCTGATCTATGCTGCTTCCACTTTACAAACTGGGGTCCCATCAAGATT21340.21945927045732677No Hit
GATCTATGCTGCTTCCACTTTACAAACTGGGGTCCCATCAAGATTCAGCGGCAGTGGCTCTGGGGCAGATTTCA21250.21853371589588538No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20940.21534569462869835No Hit
GGGTATTGGCACCTGGTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTTC20900.2149343370458355No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT20440.2102037248429128No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19360.19909707010561606No Hit
GTATTGGCACCTGGTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTTCCA18460.1898415244912021No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18370.18891596992976067No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18360.188813130534045No Hit
AGTCAGGGTATTGGCACCTGGTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATG18130.18644782443258365No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17250.1773979576096011No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17230.17719227881816965No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16630.17102191507522702No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16210.16670266045516716No Hit
GGCCAAAAGTGTATGGGGAACTGCTAGCCTGTTGACAAAAGTAAGTTGCA16210.16670266045516716No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCCTGGTCCCCTGGCCAAAAGTGTATGGGGAA16190.16649698166373575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15780.1622805664393916No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA14760.1517909480763891No Hit
TCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCT14550.14963132076635918No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14080.14479786916772075No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14020.1441808327934265No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13980.14376947521056366No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13920.1431524388362694No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13610.13996441756908237No Hit
GGTTAGCCTGGTATCAGAAGAAACCAGGGAAAGCCCCTAAACTCCTGATC13500.13883318421620955No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13230.13605652053188536No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12920.13286849926469832No Hit
GTGTATGGGGAACTGCTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTC12880.13245714168183548No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12790.1315315871203941No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12740.13101739014181552No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12680.13040035376752127No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12640.12998899618465842No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12600.12957763860179555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12500.12854924464463846No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12320.12669813552175566No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12000.12340727485885294No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11980.12320159606742151No Hit
ATGCTGCTTCCACTTTACAAACTGGGGTCCCATCAAGATTCAGCGGCAGTGGCTCTGGGGCAGATTTCACTCTCA11970.1230987566717058No Hit
GTGATGGTGAGAGTGAAATCTGCCCCAGAGCCACTGCCGCTGAATCTTGA11840.12176184452740155No Hit
GGCTGGTGATGGTGAGAGTGAAATCTGCCCCAGAGCCACTGCCGCTGAAT11810.12145332634025442No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11790.121247647548823No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCAACTTACTTTTGTCAACAGGCTAGCAGTTCC11670.12001357480023447No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11620.11949937782165593No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11570.11898518084307737No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11410.11733975051162598No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC11320.11641419595018458No Hit
GTATCAGAAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATGCTGCTT11260.11579715957589035No Hit
GTAATAGACGGCCGTGTCCGCAGCGGTCACAGACCTCAGCTTCAGGGAGA11230.11548864138874321No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11040.11353469287014469No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTTCTGATACCAGGC11040.11353469287014469No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10700.11003815341581052No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10650.10952395643723198No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTTCTGGAGCTGGATCCGGCAGCC10590.1089069200629377No Hit
CATCAAGATTCAGCGGCAGTGGCTCTGGGGCAGATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTG10510.10808420489721203No Hit
GAGTGAAATCTGCCCCAGAGCCACTGCCGCTGAATCTTGATGGGACCCCA10430.10726148973148635No Hit
ATCTTGATGGGACCCCAGTTTGTAAAGTGGAAGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTT10270.10561605940003496No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10150.10438198665144645No Hit
GCCTGGTCCCCTGGCCAAAAGTGTATGGGGAACTGCTAGCCTGTTGACAAAAGTAAGTTGCAAAATCTTCAGG9960.10242803813284793No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC9850.1012968047799751No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9740.10016557142710229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16850.039.5595551
GATAGTG700.001424418938.98317770
GTGGTAT2300.038.841421
AATGGTA1902.4738256E-1035.90555270
GCGTTAG400.002517406134.71729746
TATCAAC19500.034.1606452
ATCAACG20150.032.866293
CAACGCA20250.032.6989445
TCAACGC20550.032.2232444
AACGCAG20850.031.9225166
AGAGTAC22600.028.8434111
CGCAGAG23200.028.5411058
CAGAGTA23550.027.8255610
GCAGAGT24000.027.7326859
CTTAGAC500.00794132327.4510173
ACGCAGA24450.027.222277
CGGGTCT3850.026.59631564
GGGTCTA3850.025.75416265
GACGGGT4100.025.74042762
TTGGACG4150.025.34078259
Produced by FastQC (version 0.11.5)