FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907329
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25220.2779587117792995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12550.041.9985921
GTATCAA32400.039.4022981
TATCAAC36750.034.1531682
ATCAACG37300.033.8105623
TCAACGC37750.033.5848964
CAACGCA38250.033.1458745
TGGTATC4800.032.774712
AACGCAG39550.032.0563776
ACGCAGA41350.030.6609367
GTGGTAT5350.030.0678121
AGAGTAC42550.029.79459211
CGCAGAG42800.029.7003968
CAGAGTA43550.029.26737610
GTCCGTA500.00739321427.85603545
GCAGAGT46200.027.588629
CGACCGG950.00848356727.07910770
TACGGGC6650.026.72198715
CGAATTA1058.345414E-726.03680615
AGTACGG28950.025.37774313
GCGACCG700.001157190125.25006159
Produced by FastQC (version 0.11.5)